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Greetings,
To follow up on Aikonik's post, yes it is the same in Vista. On the right side double click on disk management. If your drive exists right click on it choose : Change Drive Letters and...

You are going to be editing the registry so incase anything goes wrong you should backup the registry:

1. Click Start, and then click Run.
2. In the Open box, type regedit, and then click OK.
3....